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| Status |
Public on Dec 05, 2019 |
| Title |
Development of gene expression signatures in colon tissues from wildtype and CARD9 knockout tumor mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of mRNA expression profile for colon tissues from wildtype and CARD9 knockout mice treated with AOM/DSS
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| Overall design |
WT and Card9-/- mice were injected intraperitoneally with AOM (10 mg/kg) on day 1. After 5 days, 2% DSS (molecular weight, 36-50 kDa) was added to the drinking water for 7 consecutive days. Three cycles of DSS treatment were used, and mice were euthanized on day 100. Colon tissues were collected from mice. mRNA of the colon tissues were isolated with TRIZOL and subject to RNA-seq
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| Contributor(s) |
Wang T, Lin X |
| Citation(s) |
30231984 |
| Submission date |
Mar 09, 2018 |
| Last update date |
Dec 05, 2019 |
| Contact name |
TINGTING WANG |
| E-mail(s) |
wangtt@nju.edu.cn
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| Organization name |
Nanjing University
|
| Street address |
22 Hankou Road
|
| City |
Nanjing |
| ZIP/Postal code |
210093 |
| Country |
China |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA437570 |
| SRA |
SRP134232 |
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