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| Status |
Public on Jul 06, 2018 |
| Title |
CRISPR-SKIP: Programmable Gene Splicing with Single Base Editors [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes in vivo by introducing mutations at target sites in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for introducing mutations at off-target sites, technologies capable of introducing targeted changes with increased precision, such as cytidine deaminase single-base editors, are preferred. We here present a versatile method termed CRISPR-SKIP that utilizes cytidine deaminase single-base editors to program de-novo exon skipping by mutating target DNA bases within splice acceptor sites. Given its simplicity and precision, CRISPR-SKIP will be broadly applicable in gene therapy and synthetic biology.
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| Overall design |
Four exons were targeted by CRISPR-SKIP. Targeted exons and their flanking exons were sequenced. Transcripts with inclusion and exclusion of targeted exon were quantified. Two control samples and two treated samples were used for each exon targeted.
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| Contributor(s) |
Gapinske M, Luu A, Winter J, Woods WS, Kostan K, Shiva N, Song JS, Perez-Pinera P |
| Citation(s) |
30107853 |
| Submission date |
Mar 09, 2018 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Alan Luu |
| E-mail(s) |
alanluu2@illinois.edu
|
| Organization name |
University of Illinois at Urbana Champaign
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| Department |
Physics
|
| Lab |
Song Lab
|
| Street address |
1110 W Green St.
|
| City |
Urbana |
| State/province |
Illinois |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE111646 |
CRISPR-SKIP: Programmable Gene Splicing with Single Base Editors |
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| Relations |
| BioProject |
PRJNA437656 |
| SRA |
SRP134380 |
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