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Series GSE111871 Query DataSets for GSE111871
Status Public on Jan 07, 2019
Title Runx/Cbfβ complexes protect ILC2s from exhaustion during allergic airway inflammation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Group 2 innate lymphoid cells (ILC2s) have tissue-resident competence and contribute to the pathogenesis of allergic diseases. Therefore, there should be mechanisms to maintain the capacity of ILC2s to produce TH2 cytokines under chronic inflammatory conditions. Here, we report that Runx proteins are essential to prevent exaggerated activation of ILC2, in part by antagonizing GATA-3 function at steady state. However, during allergic inflammation, the absence of Runx in ILC2s impaired their ability to proliferate and produce effector TH2 cytokines and chemokines, but instead induced expression of T cell exhaustion markers including IL-10 and TIGIT. These exhausted ILC2s were unabale to induce type 2 immune responses against repeated allergen inhalation. Thus, Runx proteins protect ILC2s from exhaustion during continuous allergic inflammation.
 
Overall design RNA-seq data of lung ILC2s from Cbfb +/f PLZF-Cre mice and Cbfb f/f PLZF-Cre mice (biological triplicates). RNA-seq data of cultured lung ILC2s from Cbfb +/f PLZF-Cre mice and Cbfb f/f PLZF-Cre mice (biological triplicates). ChIP-seq data of ILC2s cultured with IL-2/IL-7 or IL-2/IL-7/IL-33 for bindings of CBFb, Runx1, Runx3, GATA-3, and H3K27 acetylation (technical duplicates).
 
Contributor(s) Takashi E
Citation(s) 30683858
Submission date Mar 15, 2018
Last update date Mar 21, 2019
Contact name Takashi Ebihara
E-mail(s) tebihara@med.akita-u.ac.jp
Phone 81-188846080
Organization name Akita University Graduate School of Medicine
Department Department of Medical Biology
Street address 1-1-1 Hondo
City Akita City
ZIP/Postal code 010-8543
Country Japan
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (30)
GSM3042984 Lung_ILC2_Cbfb-het-PLZF-Cre_RNA-seq_rpt_1
GSM3042985 Lung_ILC2_Cbfb-het-PLZF-Cre_RNA-seq_rpt_2
GSM3042986 Lung_ILC2_Cbfb-het-PLZF-Cre_RNA-seq_rpt_3
Relations
BioProject PRJNA438449
SRA SRP135762

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111871_RAW.tar 561.3 Mb (http)(custom) TAR (of TXT, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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