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| Status |
Public on Jun 04, 2020 |
| Title |
A genome-wide screen identifies E2F6 as critical factor for TMZ chemoresistance in EGFRvIII expressing GBM (DNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
We conducted a genome-wide screen by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 library to identify genes which are particularly resistant to EGFRvIII GBM cells under the chemo-stress of TMZ. In vitro and in vivo validation of EGFRvIII-specific targets revealed several strong hits, including E2F6. Mechanistic studies demonstrated that E2F6 reveals its resistance to TMZ through NF-κB and TMZ inducement.
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| Overall design |
A pooled genome-wide CRISPR-Cas9-based screen using a sgRNA lentiviral library harboring 123,411 sgRNAs which target 20,914 human genes were performed to transduce EGFRwt and EGFRvIII U87 cells ( no replicate), then treated with TMZ for 7 and 14 days. The end time point of each screen was compared to day 0 in order to determine which sgRNAs were overrepresented or underrepresented in the population.
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| Contributor(s) |
Kang C |
| Citation(s) |
31508283 |
| Submission date |
Apr 05, 2018 |
| Last update date |
Jun 04, 2020 |
| Contact name |
Kai Huang |
| E-mail(s) |
huangkai@tijmu.edu.cn
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| Phone |
13752390609
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| Organization name |
tianjin medical university
|
| Street address |
anshan roud
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| City |
tianjin |
| ZIP/Postal code |
300054 |
| Country |
China |
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| Platforms (1) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE112736 |
A genome-wide screen identifies E2F6 as critical factor for TMZ chemoresistance in EGFRvIII expressing GBM |
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| Relations |
| BioProject |
PRJNA448880 |
| SRA |
SRP137675 |
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