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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 19, 2018 |
| Title |
RNA-seq transcriptional profiling in primary human hepatocyte(in vitro/in vivo) and Proliferting human hepatocyte(in vitro/in vivo) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The source of human hepatocytes (HHs) is limited because of the shortage of donor organs and the difficulty to expand them in vitro. We developed a defined medium to expand HHs for about 10,000-fold in numbers. The proliferating HHs are bi-phenotypic, showing partially maintained hepatic features and additionally gained expression of progenitor-associated genes. Importantly, proliferating HHs extensively engraft in injured mouse livers at the level comparable to primary HHs. Moreover, these cells are matured upon transplantation in vivo or differentiation in maturation medium in vitro.
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| Overall design |
During the culture process, transcriptional profiling in PHH and different passages proliHHs were generated by RNA-seq analysis. Transcriptional profiling in PHH and ProliHH-repopulated Fah-/-Rag2-/-IL2rg-/- mouse liver were generated by RNA-seq analysis.
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| Contributor(s) |
Zhang K, Zhang L, Hui L |
| Citation(s) |
30416071, 34382351 |
| Submission date |
Apr 09, 2018 |
| Last update date |
Oct 19, 2021 |
| Contact name |
Lijian Hui |
| E-mail(s) |
huilab@sibcb.ac.cn
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| Organization name |
CEMCS, CAS
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| Department |
SIBCB
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| Lab |
Huilab
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| Street address |
yueyang road 320
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA449446 |
| SRA |
SRP139248 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112866_genes.fpkm.log2.txt.gz |
3.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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