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| Status |
Public on Apr 13, 2018 |
| Title |
Sequencing of human chain specific ribosome transcriptional group |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The purpose of this study is to investigate whether the expression profile of long chain non coding RNA (lncRNA) in different concentrations of PM2.5 after treatment of normal human bronchial cells is changed. The total RNAs were extracted from 16HBE cells after exposure to either PM2.5, at concentration of 50 μg/mL and 100μg/mL, or PBS (control group) for 48h. lncRNA-sequencing, detection and analysis was performed. Fold change ≥ 2 and p ≤ 0.05 were used as a cutoff values to determine significant differential expression Further, cluster analyses were performed to classify lncRNAs based on their distinguishable expression in different samples.
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| Overall design |
Detection of a long chain noncoding RNA treated with cell type and different concentrations of PM2.5
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| Contributor(s) |
Tan Y, Wang Y, peng X |
| Citation missing |
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| Submission date |
Apr 12, 2018 |
| Last update date |
Dec 23, 2018 |
| Contact name |
Tan yi |
| E-mail(s) |
T644313012@163.com
|
| Organization name |
South China Institute of Environmental Science (SCIES), Ministry of Environmental Protection (MEP)
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| Street address |
No.7 West Street, Yuancun, Guangzhou, P. R. China
|
| City |
Guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA449927 |
| SRA |
SRP139810 |
