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| Status |
Public on Jun 30, 2019 |
| Title |
Phenotyping spermatogenic defects by single-cell expression profiling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using Drop-seq, we generated high-throughput single-cell expression data from wild-type and four mutant models with male infertility phenotype. Our study demonstrates the applicability of single-cell RNA-sequencing in study of male gonadal dysfunction and provides cell atlas resource for testis.
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| |
|
| Overall design |
Drop-seq was performed on FACS sorted germ cell populations, wild-type whole testes and mutant whole testes. Different experimental batches for wild-type and mutant strains were generated.
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| |
|
| Contributor(s) |
Jung M, Conrad D |
| Citation(s) |
31237565 |
| Submission date |
Apr 17, 2018 |
| Last update date |
Apr 29, 2022 |
| Contact name |
Donald F. Conrad |
| E-mail(s) |
conradon@ohsu.edu
|
| Organization name |
Oregon Health & Science University
|
| Department |
Division of Genetics; Oregon National Primate Research Center
|
| Street address |
505 NW 185th Ave
|
| City |
Beaverton |
| State/province |
OR |
| ZIP/Postal code |
97006 |
| Country |
USA |
| |
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| Platforms (3) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (27)
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| Relations |
| BioProject |
PRJNA450677 |
| SRA |
SRP140739 |
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