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| Status |
Public on Jan 12, 2022 |
| Title |
Peroxisome function relies on organelle-associated mRNA translation |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Crucial metabolic functions of peroxisomes rely on a variety of peroxisomal membrane proteins (PMPs). While mRNA transcripts of PMPs were shown to be colocalized with peroxisomes, the process by which PMPs efficiently couple translation with targeting to the peroxisomal membrane remained elusive. Here, we combine quantitative electron microscopy with proximity-specific ribosome profiling and reveal that translation of specific PMPs occurs on the surface of peroxisomes in the yeast Saccharomyces cerevisiae. This places peroxisomes alongside chloroplasts, mitochondria, and the endoplasmic reticulum as organelles that use localized translation for ensuring correct insertion of hydrophobic proteins into their membranes. Moreover, the correct targeting of these transcripts to peroxisomes is crucial for peroxisomal and cellular function, emphasizing the importance of localized translation for cellular physiology.
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| Overall design |
Ribosome profiling of whole cell or streptavidin-purified ribosomes biotinylated by peroxisome-localized biotin ligase in yeast.
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| Web link |
https://www.science.org/doi/full/10.1126/sciadv.abk2141?af=R
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| Contributor(s) |
Dahan N, Bykov Y, Boydston EA, Fadel A, Gazi Z, Hocberg-Laufer H, Martenson J, Denic V, Shav-Tal Y, Weissman JS, Aviram N, Zalckvar E, Schuldiner M |
| Citation(s) |
35020435 |
| Submission date |
Apr 23, 2018 |
| Last update date |
Jan 14, 2022 |
| Contact name |
Jonathan S Weissman |
| Organization name |
Whitehead Institute for Biomedical Research, MIT
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| Department |
HHMI, Biology
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| Lab |
Weissman
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| Street address |
455 Main St
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA451682 |
| SRA |
SRP142372 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE113567_RAW.tar |
650.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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