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| Status |
Public on Jun 10, 2020 |
| Title |
Genome-wide estrogen-related receptor gamma (ERRg) occupancy in human iPS cell-derived cardiomyocytes (hiPSC-CMs) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Estrogen-related receptor gamma (ERRg) has been shown to control gene expression involved in a broad range of mitochondrial energy metabolism including oxidative phosphorylation, TCA cycle, and fatty acid oxidation. However, ERRg direct targets were not identified in cardiomyocytes. With ERRg ChIP-seq, we found ERRg peaks on the promoter regions of mitochondrial energy metabolic genes as expected. Besides, ERRg extensively distributed the promoter regions of cardiac contractile, ion channels and Ca2+ handling protein genes. Surprisingly, the peaks also were found on non-cardiomyocyte genes and genes expressed in early-stage cardiomyocytes.
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| |
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| Overall design |
With ChIP-seq, examination of ERRg occupancy on the human genome in wild type hiPSC-CMs and ERRg KO hiPSC-CMs.
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| |
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| Contributor(s) |
Sakamoto T, Kim J, Won KJ, Kelly DP |
| Citation(s) |
32212902 |
| Submission date |
Apr 27, 2018 |
| Last update date |
Jun 10, 2020 |
| Contact name |
Tomoya Sakamoto |
| Organization name |
University of Pennsylvania
|
| Department |
Cardiovascular Institute
|
| Lab |
Daniel Kelly lab
|
| Street address |
Smilow Center for Translational Research 11th FL (Room 11-172) 3400 Civic Center Blvd Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE113784 |
Estrogen-related Receptor Signaling Coordinately Controls Cardiac Energy Metabolic and Structural Maturation |
|
| Relations |
| BioProject |
PRJNA453931 |
| SRA |
SRP143463 |
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