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| Status |
Public on Mar 06, 2020 |
| Title |
Genome-wide analysis of mRNA polyadenylation in Cortex/Striatum of CPEB4 modified mice [II] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To test the mRNA polyadenylation status in brain of CPEB4 modified mice we performed poly(U)-chromatography on RNA purified from a pool of cortex and striatum tissues of control and TgCPEB4∆4 mice
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| Overall design |
Control and TgCPEB4∆4 mice (n=3) were sacrificed at the age of 6 weeks. The cortex and striatum together were quickly dissected, homogenized and total RNA (INPUT) was extracted. The poly(A) RNA fraction was purified by poly(U)-chromatography. Differential elution with 25% or 90% formamide yielded two fractions of mRNAs enriched for short (WASH) or long (ELUTED) poly(A)-tails, respectively. mRNA fractions were extracted, purified and amplified. cDNA was hybridized in GeneChip Mouse PrimeView arrays and CEL files were generated from DAT files using Affymetrix Command Console software.
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| Contributor(s) |
Parras A, Anta H, Stephan-Otto Attolini C, Mendez R, Lucas JJ |
| Citation(s) |
30111840 |
| Submission date |
Apr 30, 2018 |
| Last update date |
Mar 06, 2020 |
| Contact name |
Camille Stephan Otto Attolini |
| Organization name |
IRB Barcelona
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| Department |
Biostatistics and bioinformatics Unit
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| Street address |
Baldiri Reixac 10
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| City |
Barcelona |
| State/province |
Select a State or Province |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE113842 |
Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing |
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| Relations |
| BioProject |
PRJNA454220 |
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