|
| Status |
Public on Oct 08, 2019 |
| Title |
Genome wide differential gene expression analysis of MYCN-amplified neuroblastoma cells after lncNB1 knockdown with siRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We identified that knocking lncNB1 reduces N-Myc protein stability, therefore, we performed affymetrix micoarray studies to investigate whether it was a direct or indirect effect.
lncNB1 stabilizes N-Myc protein expression through activating DEPDC1B gene transcription.
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| |
|
| Overall design |
We performed Affymetrix gene_array and Clariom_S_array in BE(2)-C cells after transfection with Control siRNA, lncNB1 siRNA-1 or lncNB1 siRNA-2 for 40 hours.
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| |
|
| Contributor(s) |
Liu T, Liu P, Mayoh C, Ho N, Peng H, Li J, Liu B |
| Citation(s) |
31690716 |
| Submission date |
May 03, 2018 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Pei Liu |
| E-mail(s) |
pliu@ccia.unsw.au
|
| Phone |
61 02 9385 3079
|
| Organization name |
CCIA
|
| Street address |
C25 Lowy Cancer Research Centre
|
| City |
Kensington |
| ZIP/Postal code |
2052 |
| Country |
Australia |
| |
|
| Platforms (2) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
|
| Samples (12)
|
| GSM3928219 |
BE(2)-C control siRNA transfected replicate 1 |
| GSM3928220 |
BE(2)-C lncNB siRNA-1 transfected replicate 1 |
| GSM3928221 |
BE(2)-C lncNB siRNA-2 transfected replicate 1 |
| GSM3928222 |
BE(2)-C control siRNA transfected replicate 2 |
| GSM3928223 |
BE(2)-C lncNB siRNA-1 transfected replicate 2 |
| GSM3928224 |
BE(2)-C lncNB siRNA-2 transfected replicate 2 |
| GSM3928225 |
BE(2)-C control siRNA transfected replicate 3 |
| GSM3928226 |
BE(2)-C lncNB siRNA-1 transfected replicate 3 |
| GSM3928227 |
BE(2)-C lncNB siRNA-2 transfected replicate 3 |
| GSM3928228 |
BE(2)-C control siRNA transfected replicate 4 |
| GSM3928229 |
BE(2)-C lncNB siRNA-1 transfected replicate 4 |
| GSM3928230 |
BE(2)-C lncNB siRNA-2 transfected replicate 4 |
|
| Relations |
| BioProject |
PRJNA454830 |
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