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| Status |
Public on Nov 28, 2018 |
| Title |
Gene expression data in mouse whole lung |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Telomere dysfunction in type II AECs, mediated by deletion of the telomere shelterin protein TRF1, leads to spontaneous development of pulmonary fibrosis in mice (SPC-creTRF1flox/flox mice). We sought to identify gene signature in these lungs in an effort to understand molecular mechanisms of the disease.
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| Overall design |
Tamoxifen was administered to SPC-creTRF1flox/flox mice and TRF1flox/flox littermate controls beginning at 8 weeks age. Lungs from SPC-creTRF1flox/flox mice were collected after 3 months and 9 months of tamoxifen administration whereas lungs from TRF1flox/flox controls were collected after 9 months of tamoxifen administration. This design is intended to identify gene changes at both early and late stage of the disease and compare with controls. Mouse whole lungs were harvested for RNA extraction and hybridization on one color Agilent microarrays.
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| Contributor(s) |
Naikawadi RP, Wolters PJ |
| Citation(s) |
30643263 |
| Submission date |
May 03, 2018 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Paul John Wolters |
| E-mail(s) |
paul.wolters@ucsf.edu
|
| Phone |
415-514-2601
|
| Organization name |
University of California, San Francisco
|
| Department |
Pulmonary, Critical Care, Allergy and Sleep Medicine
|
| Street address |
513 Parnassus Ave HSE 1355B
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (9)
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| GSM3130460 |
Ms Lung_TRF1flox/flox 9 Mon Tamox_biological rep1 |
| GSM3130461 |
Ms Lung_TRF1flox/flox 9 Mon Tamox_biological rep2 |
| GSM3130462 |
Ms Lung_TRF1flox/flox 9 Mon Tamox_biological rep3 |
| GSM3130463 |
Ms Lung_SPC-cre TRF1flox/flox 3 Mon Tamox_ biological rep1 |
| GSM3130464 |
Ms Lung_SPC-cre TRF1flox/flox 3 Mon Tamox_ biological rep2 |
| GSM3130465 |
Ms Lung_SPC-cre TRF1flox/flox 3 Mon Tamox_ biological rep3 |
| GSM3130466 |
Ms Lung_SPC-cre TRF1flox/flox 9 Mon Tamox_ biological rep1 |
| GSM3130467 |
Ms Lung_SPC-cre TRF1flox/flox 9 Mon Tamox_ biological rep2 |
| GSM3130468 |
Ms Lung_SPC-cre TRF1flox/flox 9 Mon Tamox_ biological rep3 |
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| Relations |
| BioProject |
PRJNA454862 |
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