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Series GSE114054 Query DataSets for GSE114054
Status Public on May 05, 2018
Title Drosophila CNS mitochondrial dysfunction with Ras/MAPK inhibition microarray
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Mitochondrial dysfunction causes biophysical, metabolic and signalling changes that alter homeostasis and reprogram cells. We used a Drosophila model in which TFAM is overexpressed in the nervous system with or without Ras/MAPK pathway inhibition, by knock-down of the ETS transcription factor pointed, to investigate the how mitochondrial dysfunction and Ras/MAPK signalling affect the transcriptome.
We used microarray analysis to investigate gene expression in cases of mitochondrial dysfunction in the CNS with or without Ras/MAPK pathway inhibition by knock-down of pointed (Pnt) and anterior open (Aop).
 
Overall design RNA was purified from the late third instar larval CNS from control larvae, or larvae over-expressing mitochondrial transcription factor A (TFAM) in post-mitotic neurons using the neuron specific driver nSyb-Gal4, or larvae over-expressing TFAM in combination with knock-down of Pnt or Aop. nSyb-Gal4 driving Pnt or Aop knock-down alone conditions were also included. Three replicates are included for all conditions.
 
Contributor(s) Bateman JM, Duncan OF
Citation(s) 30059502
Submission date May 04, 2018
Last update date Aug 13, 2018
Contact name Joseph M. Bateman
E-mail(s) joseph_matthew.bateman@kcl.ac.uk
Phone 2078488144
Organization name King's College London
Street address Guy's campus
City London
State/province (Click to Select US State)
ZIP/Postal code SE1 1UL
Country United Kingdom
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (18)
GSM3131611 control rep1
GSM3131612 control rep2
GSM3131613 control rep3
Relations
BioProject PRJNA463815

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114054_RAW.tar 32.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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