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| Status |
Public on Mar 05, 2019 |
| Title |
Exosome release is regulated by mTORC1 via a Rab27A-dependent mechanism |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Exosomes are small membrane-bound vesicles released into extracellular spaces by many types of cells. These nanovesicles carry proteins, mRNA and miRNA and are involved in cell waste management and intercellular communication. In the present study we show that exosome release, which leads to net loss of cellular membrane and protein content, is negatively regulated by mechanistic target of rapamycin complex 1 (mTORC1). We find that in cells and animal models exosome release is inhibited by sustained activation of mTORC1, leading to intracellular accumulation of CD63-positive exosome precursors. Inhibition of mTORC1 by rapamycin or nutrient and growth factor deprivation stimulates exosome release, which occurs concomitantly with autophagy. The drug-stimulated release is blocked by siRNA-mediated downregulation of small GTPase Rab27A. Analysis of the cargo content in exosomes released from rapamycin treated cells reveals that inhibition of mTORC1 does not alter its protein and miRNA profiles. These observations demonstrate that exosome release, like autophagy, is negatively regulated by mTORC1 in response to changes in nutrient and growth factor conditions
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| Overall design |
Examination of 2 different microRNA expressions on rapamycin (Rapa) or mock treated (Ctrl) cells
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| Contributor(s) |
Zou W, Bai X |
| Citation(s) |
30775228 |
| Submission date |
May 09, 2018 |
| Last update date |
Mar 05, 2019 |
| Contact name |
zou wen chong |
| E-mail(s) |
zouwenchong1105@163.com
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| Phone |
+8615622733530
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| Organization name |
Southern Medical University
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| Department |
Department of Cell Biology
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| Lab |
State Key Laboratory of Organ Failure Research
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| Street address |
Sha Tai ROAD
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| City |
guangzhou |
| State/province |
guangdong |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA470637 |
| SRA |
SRP145074 |
