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Series GSE11430 Query DataSets for GSE11430
Status Public on Jun 27, 2008
Title Performance comparison of Affymetrix and Illumina microarray technologies_AffymetrixDataset
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The present study was conducted to compare the ability of Affymetrix and Illumina microarray technologies to characterize the differential gene expression profiles of human monocytes and monocyte-derived-macrophages.
Keywords: Type Cross-platform comparison Summary
Overall design Blood samples were obtained from patients with symptoms of acute coronary syndrome who had undergone coronary angiography at the department of cardiology of the Pitié-Salpêtrière Hospital, Paris and who had one stenosis >50 % diagnosed in at least one major coronary artery.
RNA samples were extracted from monocyte and monocyte derived macrophage cells. Five biological replicates for each type of sample were hybridized in parallel to the Affymetrix HGU133 plus 2.0 and Illumina Bead Chip Human-6 v1 platforms. In addition, a reference list of differentially expressed genes was generated from a larger number of hybridizations (mRNA from 86 individuals) using a third technology (the academic RNG/MRC two-color chip) and was used as reference to assess the performance of the 2 platforms based on 10 samples. The internal consistency of each platform, the overlap in lists of genes identified as differentially expressed generated on each array type and the biological relevance of significant genes identified on each platform (Gene Ontology (GO) classes enrichments) were assessed.
Contributor(s) Maouche S, Poirier O, Godefroy T, Olaso R, Gut I, Collet J, Montalescot G, Cambien F
Citation(s) 18578872
Submission date May 13, 2008
Last update date Mar 25, 2019
Contact name Francois Cambien
Fax (33) 140779728
Organization name INSERM
Department Cardiovascular Genomics
Street address 91 Bd de l'Hôpital
City Paris
State/province France
ZIP/Postal code 75634 Paris Cedex 13
Country France
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (10)
GSM257664 Affy_Monocyte_16
GSM257665 Affy_Macrophage_16
GSM257666 Affy_Monocyte_20
This SubSeries is part of SuperSeries:
GSE11540 Performance comparison of Affymetrix and Illumina microarray technologies
BioProject PRJNA109231

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11430_RAW.tar 48.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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