|
| Status |
Public on Sep 21, 2018 |
| Title |
CD95L mRNA is toxic to cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
>80% of a large number of tested siRNAs and shRNAs targeting CD95 or CD95 ligand (CD95L) induce a form of cell death that is characterized by the simultaneous activation of multiple death pathways and preferentially affects transformed and cancer stem cells. We now show that these si/shRNAs kill cancer cells through canonical RNAi by targeting the 3’UTR of critical survival genes in a unique form of off-target effect. We also provide evidence showing that the full length CD95L mRNA is also toxic and produces small Ago-associated RNAs.
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| |
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| Overall design |
Pan Argonaute immunoprecipitation and small RNA cloning in HeyA8 and HCT116 wild-type and HCT116 Drosha KO cells treated with plenti or hFasL
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| |
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| Contributor(s) |
Putzbach WE, Haluck-Kangas A, Gao QQ, Sarshad AA, Bartom E, Stults A, Qadir AS, Scholtens DM, Hafner M, Peter ME |
| Citation(s) |
30324908 |
| Submission date |
May 14, 2018 |
| Last update date |
Dec 23, 2018 |
| Contact name |
Markus Hafner |
| E-mail(s) |
Markus.hafner@nih.gov
|
| Phone |
3014026956
|
| Organization name |
NIH
|
| Department |
NIAMS
|
| Lab |
Markus Hafner
|
| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA471325 |
| SRA |
SRP145632 |
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