|
| Status |
Public on May 14, 2019 |
| Title |
RNA sequencing in hepatocytes and DR-derived hepatocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
During chronic liver injury, the ductular reaction generates hepatocytes. The aim of the analysis is to compare the phenotype of the DR-derived hepatocytes with that of native hepaotcytes in the same injured liver and control healthy hepatocytes.
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| Overall design |
We compared 5 biological replicates of each of the 3 populations. Due to technical constraints 10 samples were processed in a first experiment (Experiment 1) and 9 samples were processed in a second experiment (experiment 2). Log fold-change obtained from both experiment were highly correlated (r ~ 0.95).
Several of the same samples were processed in both Experiment 1 and Experiment 2: CTL 5, RED 2, RED 3, RED 4. For these samples, independent libraries were made for each Experiment following methods outlined in the Extraction protocol on the sample pages.
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| Contributor(s) |
Ambroise J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 14, 2018 |
| Last update date |
May 14, 2019 |
| Contact name |
Jérôme Ambroise |
| E-mail(s) |
jambroise83@gmail.com
|
| Organization name |
Université catholique de Louvain
|
| Department |
Institut de Recherche Expérimentale et Clinique
|
| Street address |
Avenue Hippocrate 54
|
| City |
Woluwé |
| ZIP/Postal code |
1200 |
| Country |
Belgium |
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| Platforms (2) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (19)
|
| GSM3141686 |
Experiment 1 native Healthy hepatocytes - CTL 3 |
| GSM3141687 |
Experiment 1 native Healthy hepatocytes - CTL 4 |
| GSM3141688 |
Experiment 1 native Healthy hepatocytes - CTL 5 |
| GSM3141689 |
Experiment 1 Native tdTomato hepatocytes – RED 1 |
| GSM3141690 |
Experiment 1 Native tdTomato hepatocytes – RED 2 |
| GSM3141691 |
Experiment 1 Native tdTomato hepatocytes – RED 3 |
| GSM3141692 |
Experiment 1 Native tdTomato hepatocytes – RED 4 |
| GSM3141693 |
Experiment 1 Native tdTomato hepatocytes – RED 5 |
| GSM3141694 |
Experiment 1 DR-derived hepatocytes – GREEN 4 |
| GSM3141695 |
Experiment 1 DR-derived hepatocytes – GREEN 5 |
| GSM3141696 |
Experiment 2 native Healthy hepatocytes - CTL 1 |
| GSM3141697 |
Experiment 2 native Healthy hepatocytes - CTL 2 |
| GSM3141698 |
Experiment 2 native Healthy hepatocytes - CTL 5 |
| GSM3141699 |
Experiment 2 Native tdTomato hepatocytes – RED 2 |
| GSM3141700 |
Experiment 2 Native tdTomato hepatocytes – RED 3 |
| GSM3141701 |
Experiment 2 Native tdTomato hepatocytes – RED 4 |
| GSM3141702 |
Experiment 2 DR-derived hepatocytes – GREEN 1 |
| GSM3141703 |
Experiment 2 DR-derived hepatocytes – GREEN 2 |
| GSM3141704 |
Experiment 2 DR-derived hepatocytes – GREEN 3 |
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| Relations |
| BioProject |
PRJNA471341 |
| SRA |
SRP145671 |
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