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| Status |
Public on Oct 01, 2008 |
| Title |
Differential SAGE analysis in Arabidopsis uncovers increased transcriptome complexity in response to low temperature |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by SAGE
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| Summary |
Five SAGE libraries were generated from A. thaliana leaf tissue collected at time points ranging from 30 minutes to one week of low temperature treatment (4°C). Over 240,000 high quality SAGE tags, corresponding to 16,629 annotated genes, provided a comprehensive survey of changes in the transcriptome in response to low temperature, from perception of the stress to acquisition of freezing tolerance.
Keywords: SAGE; time course; stress response; cold acclimation; freezing tolerance
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| Overall design |
SAGE libraries were generated from A. thaliana leaf tissue collected after exposure to low temperature (4°C) for 0 minutes, 30 minutes, 2 hours, 2 days and one week, the tags were matched to the Arabidopsis genome and statistical analysis was performed to reveal differential gene expression.
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| Contributor(s) |
Robinson SJ |
| Citation(s) |
18808718 |
| Submission date |
May 15, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Stephen James Robinson |
| E-mail(s) |
robinsons@agr.gc.ca
|
| Phone |
306-956-2843
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| Fax |
306-9567247
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| Organization name |
Agriculture and agri-Food Canada
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| Department |
Molecular Genetics
|
| Street address |
107 Science Place
|
| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7H 0X2 |
| Country |
Canada |
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| Platforms (1) |
| GPL25 |
SAGE:10:NlaIII:Arabidopsis thaliana |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA106377 |
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