|
| Status |
Public on Nov 03, 2018 |
| Title |
Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial
transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region- and tissue-specific genes.
We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.
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| |
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| Overall design |
Spatial transcriptomics by RNA-tomography (TomoSeq) on 20um sections
|
| Web link |
https://www.biorxiv.org/content/early/2018/06/15/348201
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| |
|
| Contributor(s) |
Ebbing A, Vertesy A, Betist M, Spanjaard B, Junker JP, Berezikov E, van Oudenaarden A, Korswagen HC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 21, 2018 |
| Last update date |
Nov 05, 2018 |
| Contact name |
Abel Vertesy |
| Organization name |
Hubrecht Institute
|
| Lab |
Alexander van Oudenaarden
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA472377 |
| SRA |
SRP148595 |
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