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| Status |
Public on May 20, 2021 |
| Title |
Next Generation Sequencing Analysis of human pluripotent stem cells derived endothelial and smooth muscle cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: global gene expression profiling of H1 and iPSC derived endothelial cells (ECs) and smooth mucle cells (SMCs).
Methods: SMART-seq2 amplified polyA RNA from undifferentiated H1 cells and iPSCs (in biological duplicates), cardiovascular progenitor cells, endothelial cells (ECs) and smooth muscle cells (SMCs)
Results: Insulin free condition promoted cardiovascular mesoderm, endothelial and smooth muscle differentiation.
Conclusions: the gene expression profiles of ECs and SMCs differentiated in insulin free medium confirmed that they are true ECs and SMCs.
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| Overall design |
Perform RNA-seq of undifferentaited H1 and human iPSCs, day 3 mesoderm progenitor cells, day 8 endothelial and smooth muscle cells.
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| |
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| Contributor(s) |
Na J, Zhang F, Huang R |
| Citation(s) |
33618219 |
| Submission date |
May 22, 2018 |
| Last update date |
Aug 20, 2021 |
| Contact name |
Jie Na |
| E-mail(s) |
jie.na@tsinghua.edu.cn
|
| Phone |
+86-18618192150
|
| Organization name |
Tsinghua University
|
| Department |
Basic Medical Sciences, School of Medicine
|
| Lab |
D115 School of Medicine
|
| Street address |
Tsinghua University
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA472610 |
| SRA |
SRP148697 |
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