|
| Status |
Public on May 14, 2019 |
| Title |
The transgenerational effects of mitochondrial supplementation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine the outcomes of offspring produced from mitochondrial supplementation to mature oocytes at the time of fertilisation, we generated three generations of offspring. Primordial follicles of the third generation female mice (postnatal day 3) were collected, and RNA sequencing was performed. We compared the treated and control gene expression data to determine differentially expressed genes between the two groups. Our data suggest that mitochondrial supplementation has transgenerational effects on offspring.
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| Overall design |
1. mitochondrial supplementation at the time of fertilisation was performed 2. three generations of offspring were produced to determine the transgeneration effects of the technique. 3. Primordial follicles of the third generation day 3 postnatal offspring were collected and extracted to compare the transcripts of treated vs control primordial follicles.
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| Contributor(s) |
St. John J |
| Citation(s) |
31040316 |
| Submission date |
May 29, 2018 |
| Last update date |
May 14, 2019 |
| Contact name |
Justin C St. John |
| E-mail(s) |
justin.stjohn@hudson.org.au
|
| Phone |
+61399024749
|
| Organization name |
Hudson Institute of Medical Research
|
| Department |
Centre for Genetic Diseases
|
| Lab |
Mitochondrial Genetics
|
| Street address |
27-31 Wright St
|
| City |
Clayton |
| State/province |
Vic |
| ZIP/Postal code |
3168 |
| Country |
Australia |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA473442 |
| SRA |
SRP149128 |
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