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| Status |
Public on Aug 30, 2018 |
| Title |
Single cell RNA sequencing of aortic CD45+ cells and foam cells from atherosclerotic aorta |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In atherosclerosis, several immune cells are involved in plaque formation. Foam cell formation is a major cellular process in atherosclerotic lesion. It is important to understand which cells participate in foam cell formation. To characterize the immune cells and foam cells in atherosclerotic aorta, we performed single cell RNA sequencing of aortic CD45+ leukocytes from Ldlr-/- mice and foamy cells from ApoE-/- mice. The single cell RNA-seq analyses revealed the heterogeneity of aortic macrophages and foam cells in atherosclerotic aorta.
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| Overall design |
Single cell RNA sequencing of aortic CD45+ cells from atherosclerotic Ldlr-/- mice (n=6, WD for 12 wks). 3781 cells identified, Using Illumina HiSeq4000.
Single cell RNA sequencing of aortic foam cells from atherosclerotic ApoE-/- mice (n=6, WD for 27 wks), 809 cells identified, Using Illumina HiSeq4000.
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| Contributor(s) |
Kim K, Shim D, Lee JS, Zaitsev K, Williams JW, Randolph GJ, Artyomov MN, Cheong C, Choi J |
| Citation(s) |
30359200 |
| Submission date |
Jun 25, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Maxim Artyomov |
| E-mail(s) |
martyomov@wustl.edu
|
| Organization name |
WASHINGTON UNIVERSITY IN ST LOUIS
|
| Department |
Pathology & Immunology
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| Lab |
Artyomov lab
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| Street address |
425 S Euclid Ave
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| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
| GSM3215435 |
Total leukocytes from Ldlr -/- aorta (Single cell RNA-seq) |
| GSM3215436 |
Total foam cells from ApoE -/- aorta (Single cell RNA-seq) |
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| This SubSeries is part of SuperSeries: |
| GSE116271 |
Transcriptome analysis reveals non-foamy rather than foamy plaque macrophages are pro-inflammatory in atherosclerotic murine models |
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| Relations |
| BioProject |
PRJNA477843 |
| SRA |
SRP151294 |
