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| Status |
Public on Jul 03, 2018 |
| Title |
RELMa induced differential gene expression in CD11c+ cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Mouse lung CD11c+ cell gene expression changes following recombinant RELMa treatment
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| Overall design |
RELMa-/- mice were infected with 500 L3 of Nippostrongylus brasiliensis worms and sacrificed at day 10 post infection. Lungs were collected, dissociated and sorted for CD11c+ cells. Cells were plated overnight at 37 deg C in 10% serum media. Cells were serum starved in 1% serum media for 2 hour and treated with 500ng/ml recombinant RELMa or PBS control. Cells were lysed with lysis buffer and an volume equivalent to 1000 cells per microliter was frozen at -80 deg C for NanoString analysis. Five microliter of each sample (~5000 cells) was applied to NanoString.
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| |
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| Contributor(s) |
Nair MG |
| Citation(s) |
29992625 |
| Submission date |
Jul 02, 2018 |
| Last update date |
Oct 09, 2018 |
| Contact name |
Meera Goh Nair |
| E-mail(s) |
Meera.Nair@medsch.ucr.edu
|
| Phone |
951-827-7734
|
| Organization name |
University of California Riverside
|
| Department |
Division of Biomedical Sciences
|
| Street address |
301 School of Medicine Research Building, 900 University Avenue,
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL25266 |
NanoString Mouse nCounter Myeloid Innate Immunity Panel NS_Mm_Myeloid_V2.0 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA478997 |
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