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Series GSE116900 Query DataSets for GSE116900
Status Public on Jul 11, 2018
Title RNA Seq of wild-type and VPS35-D620N-expressing SH-SY5Y cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary SH-SY5Y were created to stably express wild-type or VPS35-D620N (CMV-promoter driven) using a lentiviral vector (Vira power). Non-transduced cells were eliminated with blasticidin resistance gene included in viral cassette
 
Overall design Wild-type VPS35 and VPS35 D620N-expressing SH-SY5Y cells were differentiated with 10uM all-trans retinoic acid for 7 days and RNA extracted using Qiagen RNEasy kit. There are 3 independent experiments (replicates 1-3)
 
Contributor(s) Morrison B, Rahman A
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 10, 2018
Last update date Jan 25, 2019
Contact name Brad E Morrison
E-mail(s) bradmorrison@boisestate.edu
Organization name Boise State University
Department Biological Sciences
Street address 1910 university dr., mail stop: 1515
City Boise
State/province ID
ZIP/Postal code 83725
Country USA
 
Platforms (1)
GPL15520 Illumina MiSeq (Homo sapiens)
Samples (6)
GSM3263528 WT-rep1
GSM3263529 WT-rep2
GSM3263530 WT-rep3
Relations
BioProject PRJNA480517
SRA SRP152986

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE116900_RAW.tar 21.7 Mb (http)(custom) TAR (of BED)
GSE116900_RNA_Seq_expression_list.xlsx 3.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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