|
| Status |
Public on Oct 01, 2018 |
| Title |
RNAseq quantitative analysis of ion channel mRNA expression in microbead occlusion model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The purpose of this study was to utilize RNAseq to determine if there are changes in ion channel mRNA expression in the retina of C57BL/6 mice subjected to the Microbead Occlusion Model. Whole retina was isolated from 2 month old mice that received intracameral injections of either saline or 15-um-diameter microbeads. Total RNA was isolated from the retinas and subjected to RNA sequencing using Illumina HiSeq 2500. Fold change in mRNA expression was compared between saline- and microbead-injected samples. We then analyzed these data for changes in expression of potassium channels, such as tandem-pore domain K+ channels, inwardly rectifying K+ channels, and the Na+/K+-ATPase.
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| |
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| Overall design |
Retinal mRNA profiles of saline- and microbead-injected C57BL/6 mice were generated by whole genome sequencing using Illumina NovaSeq6000
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| |
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| Contributor(s) |
Sappington RM, Fischer RA |
| Citation(s) |
31736686 |
| Submission date |
Jul 11, 2018 |
| Last update date |
Nov 25, 2019 |
| Contact name |
Lauren Katie Wareham |
| E-mail(s) |
lauren.wareham@vanderbilt.edu
|
| Phone |
8575408019
|
| Organization name |
Vanderbilt University Medical Center
|
| Department |
Vanderbilt Eye Institute
|
| Lab |
Sappington Lab
|
| Street address |
2213 Garland Avenue, MRBIV 11435a
|
| City |
Nashville |
| State/province |
Tennessee |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA480596 |
| SRA |
SRP153029 |
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