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| Status |
Public on Mar 20, 2019 |
| Title |
The analysis of gene expression profiles of splenocytes from wild type and UBL4A/GdX-/Y mice upon LPS stimulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To compared the gene expression profiles of splenocytes from wild type and UBLA4/GdX (Ubiquitin-like protein 4A, also named GdX) knockout mice upon LPS stimulation.
Methods: GdX+/Y and GdX-/Y mice were injected with LPS. Their splenocytes were collected at different time (90min or 6 hrs), and then performed RNA-sequencing analysis. We sequence the samples used BGISEQ-500 platform.
Results: The average mapping ratio with reference genome is 93.42%, the average mapping ratio with gene is 83.40%; A total of 17,920 genes were detected.A number of NF-κB targeting genes, such as IL-6, IL-1a and IL-12, have dramatic higher expressions in the splenocytes of GdX+/Y mice than that of GdX-/Y mice. Gene ontology (GO) analysis of GdX function in LPS-treated mice showed one of the most significantly enriched biological processes is related to inflammatory response.
Conclusions: Our study revealed the gene expression profiles of the splenocytes from LPS-treated wild type and GdX-/Y mice.
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| Overall design |
mRNA profiles of splenocytes from four wild type and three GdX-/- mice were generated by RNA-sequencing analysis
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| Contributor(s) |
Zhou Y, Liu C, Yang L |
| Citation(s) |
30867837 |
| Submission date |
Jul 11, 2018 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Yifan Zhou |
| E-mail(s) |
yfan_zhou@sina.com
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| Organization name |
Tsinghua University
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| Department |
School of Medicine
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| Street address |
No. 30 Shuangqing Road
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| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA480691 |
| SRA |
SRP153088 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE116956_AllSamples.GeneExpression.FPKM.xls.gz |
3.4 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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