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| Status |
Public on Jul 13, 2018 |
| Title |
Identification of differentially expressed genes between control and PIK3CD GOF human activated transitional B cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
IL-21 induces B cell activation, and differentiation into antibody-secreting plasmblasts in vitro. This process is compromised in transitional B cells to gain of function mutations in PIK3CD
We used microarrays to identify genes that are differentiatlly expressed following CD40L/IL-21 stimulation of human transitonal B cells from healthy donors and patients with GOF mutations in PIK3CD
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| Overall design |
Sort-purified transitional B cells from 3 healthy donors and 3 patients with APDS due to heterozygous activating gain of function PIK3CD mutations were cultured for four days with CD40L +/- IL-21 (50ng/ml). RNA was then extracted from the harvested cells and the genetic profile analysed by microarray.
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| Contributor(s) |
Tangye S |
| Citation(s) |
30018075 |
| Submission date |
Jul 12, 2018 |
| Last update date |
Feb 01, 2019 |
| Contact name |
Stuart Tangye |
| E-mail(s) |
s.tangye@garvan.org.au
|
| Phone |
+61 2 9295 8455
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| Organization name |
Garvan Institute of Medical Research
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| Department |
Immunology
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| Street address |
384 Victoria St
|
| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA480833 |
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