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| Status |
Public on Dec 16, 2018 |
| Title |
ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2 [DNA-Seq validation of KO] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
In order to investigate changes upon ZBTB10 depletion, we used CRISPR/Cas9 to induce a ZBTB10 knockout in HEK293 and U2OS cells.
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| Overall design |
ZBTB10 knockouts were generated using three different guide RNAs targeting the second exon of the ZBTB10 gene. HEK293 and U2OS cells were selected in 750 ng/mL puromycin after transfection and gDNA was extracted. A circa 1100 bp long target sequence around the cut locus was amplified by PCR and Cas9 activity was tested in a T7 endonuclease assay. After confirming Cas9 activity, single cell sorting was performed and cells were expanded to screen for ZBTB10 knockout clones by Western blot using an anti-ZBTB10 antibody (Supplementary Table 1).
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| Contributor(s) |
Bluhm A, Levin M |
| Citation(s) |
30629181 |
| Submission date |
Jul 17, 2018 |
| Last update date |
Mar 12, 2019 |
| Contact name |
Falk Butter |
| E-mail(s) |
f.butter@imb-mainz.de
|
| Organization name |
Institute of Molecular Biology gGmbH
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| Department |
Quantitative Proteomics
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| Lab |
Falk Butter
|
| Street address |
Ackermannweg 4
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| City |
Mainz |
| State/province |
Germany |
| ZIP/Postal code |
55128 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE117214 |
ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2 |
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| Relations |
| BioProject |
PRJNA481472 |
| SRA |
SRP154126 |
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