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Series GSE11722 Query DataSets for GSE11722
Status Public on Aug 01, 2008
Title Irinotecan-induced gene expression changes in the rat intestine
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The regional specificity and timing of gene activation following chemotherapy, and how this relates to subsequent mucositis development is currently unknown. The aim of the study was therefore to determine the early time course of gene expression changes along the gastrointestinal tract (GIT) of the DA rat following irinotecan treatment, so as to provide an insight into the genetic component of mucositis.
We have found that changes in gene expression following irinotecan occur by 1 hour, and persist for at least 72 h after treatment. Overall changes in gene expression are similar along the GIT, however there is temporal variability between regions.

Keywords: Time course and tissue comparison
Overall design Thirty female DA rats were placed in groups (n = 6) and treated with 200 mg/kg i.p. of irinotecan. Animals were killed at 0, 1, 6, 24 and 72 h and samples of stomach, jejunum and colon collected. These times were chosen to represent very early responses to treatment, up to the time of peak damage and morphological changes. The 0 h group did not receive irinotecan and acted as experimental controls. RNA was extracted from whole tissue samples. RNA was pooled into 2 per group (n = 3) to allow for replication of experiment. Each sample was then hybridised to Affymetrix GeneChip Arrays (Rat 230 2.0).
Contributor(s) Bowen JM, Gibson RJ, Tsykin A, Stringer AM, Logan RM, Keefe DM
Citation(s) 17594691
Submission date Jun 09, 2008
Last update date Jul 31, 2017
Contact name Joanne Marie Bowen
Organization name Royal Adelaide Hospital
Department Medical Oncology
Lab Mucositis Research
Street address North Terrace
City Adelaide
State/province South Australia
ZIP/Postal code 5000
Country Australia
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (30)
GSM297524 stomach_0h_rep1
GSM297525 stomach_0h_rep2
GSM297526 stomach_1h_rep1
BioProject PRJNA106019

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Supplementary file Size Download File type/resource
GSE11722_RAW.tar 77.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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