|
| Status |
Public on Jun 04, 2019 |
| Title |
Fucntional genetic variants mediate their regulatory effects through altered transcription factor binding. [Bisulfite-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
The extensive molecular characterization of the transcriptional regulatory landscape within the LCLs of a 3-generation, 17-member kindred was performed to allow for the identification of functional variants. A variant associated with changes in multiple molecular phenotypes was selected for validation through precise CRISPR/Cas9 editing. Edited clones were subsequently tested for reconstitution of function through CRISPR/dCas9 targeted TF activation.
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| |
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| Overall design |
DNA methylation within a 3-generation,17-member kindred were assayed using a strand sensitive library construction
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| |
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| Contributor(s) |
Johnston AD, Simoes-Pires CA, Thompson TV, Suzuki M, Greally JM |
| Citation(s) |
31375681, 31428700 |
| Submission date |
Jul 23, 2018 |
| Last update date |
Aug 27, 2019 |
| Contact name |
Andrew Johnston |
| E-mail(s) |
Andrew.Johnston@med.einstein.yu.edu
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Genetics
|
| Lab |
Price 314
|
| Street address |
1301 Morris Park Avenue
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
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| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE117576 |
Fucntional genetic variants mediate their regulatory effects through altered transcription factor binding. |
|
| Relations |
| BioProject |
PRJNA482544 |
| SRA |
SRP154980 |
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