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| Status |
Public on Jul 23, 2021 |
| Title |
Investigating the role of SMCHD1 in regulating the chromatin states of select autosomal gene clusters |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To investigate the effect of Smchd1 ablation on the chromatin states of select autosomal gene clusters, we derived clonal neural progenitor cells (NPCs) from Smchd1+/+ (wild-type, WT) and Smchd1-/- mouse embryonic stem cells (ES cells; ESC). We generated allele-specific EZH2 ChIP-seq datasets from WT and Smchd1-/- NPCs to examine the effect of Smchd1 ablation on the distribution of Polycomb repressive complex 2 (PRC2).
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| Overall design |
ChIP-seq for EZH2 in neural progenitor cell clones derived from WT and Smchd1-/- female mouse embryonic stem cells (ES cells).
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| Contributor(s) |
Wang C, Lee JT |
| Citation missing |
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| Submission date |
Jul 25, 2018 |
| Last update date |
Jul 24, 2021 |
| Contact name |
Chen-Yu Wang |
| E-mail(s) |
cywang@molbio.mgh.harvard.edu
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| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
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| Lab |
Jeannie T. Lee
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| Street address |
185 Cambridge Street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA482813 |
| SRA |
SRP155183 |
