NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE117750 Query DataSets for GSE117750
Status Public on Mar 09, 2020
Title TBX1-FGF11 as a novel axis governing limb development
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although intensively studied, the gene networks regulating limb development is still incomplete. Indeed, efforts to decipher the genetic and transcriptional control of limb formation rely mainly on transcriptome approaches obtained from embryos collected 0.5 or 1 day apart from E9.5 to E13.5. This approach identified gene networks tightly regulated during limb morphogenesis as well as novel genes possibly involved in these networks. However, given the complexity and speed of the underlying molecular processes at these stages, relying on time points 1 or even 0.5 day apart limits our ability to capture regulatory changes taking place at a faster pace, and to identify the factors on which they depend.
To obtain a comprehensive view of the limb developmental progression at the molecular level, we used the roller system to culture E10.5 and E12.5 mouse embryos over a 6h time window and performed a microarray analysis (Affymetrix GeneChip® Mouse Exon 1.0 ST Arrays) on the collected samples
 
Overall design The forelimbs of E10.5 from non-cultured mouse embryos and the forelimbs from E10.5 and E12.5 mouse embryos cultured for 1, 3 or 6 hours were collected for RNA extraction and hybridization on Affymetrix GeneChip® Mouse Exon 1.0 ST Arrays according to Affymetrix recommendations.
 
Contributor(s) Tejedor G, Laplace-Builhé B, Luz-Crawford P, Assou S, Barthelaix A, Kissa K, Jorgensen C, Collignon J, Chuchana P, Djouad F
Citation(s) 32107392
Submission date Jul 26, 2018
Last update date Mar 09, 2020
Contact name Said Assou
E-mail(s) said.assou@inserm.fr
Organization name University Hospital of Montpellier, Inserm
Department Institute for Regenerative Medecine and Biotherapy
Street address 80, av. Augustin Fliche
City Montpellier
ZIP/Postal code 34295
Country France
 
Platforms (1)
GPL6096 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]
Samples (21)
GSM3308205 Forelimbs of E10.5 from non-cultured embryo, biological rep1
GSM3308206 Forelimbs of E10.5 from non-cultured embryo, biological rep2
GSM3308207 Forelimbs of E10.5 from non-cultured embryo, biological rep3
Relations
BioProject PRJNA483116

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117750_RAW.tar 472.1 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap