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| Status |
Public on Nov 14, 2018 |
| Title |
The effect of cell culture on age-related transcriptomic changes in dermal fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
During aging, stromal functions are thought to be impaired, yet little is known whether this stems from molecular and cellular changes of fibroblasts, a major component of stroma. Using population- and single-cell whole transcriptomics, and long-term lineage tracing, we studied alterations in murine dermal fibroblasts during physiological aging under different dietary regimes known to affect longevity. We show that the identity of aged fibroblasts becomes undefined, with the distinct fibroblast states present in young skin no longer clearly demarcated. In addition, old fibroblasts not only reduce the expression of genes involved in the formation of the extracellular matrix, but intriguingly, also gain adipogenic traits, paradoxically becoming similar to neonatal pro-adipogenic fibroblasts. These age-related alterations are sensitive to systemic changes in metabolism: long-term caloric restriction prevents them in old fibroblasts in a reversible manner, whereas a high-fat diet potentiates them in young fibroblasts. Finally, inhibition of the master regulator of adipogenesis, PPARgamma, attenuates fibroblast aging in vivo, providing potential anti-aging therapeutic alternatives to caloric restriction.
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| Overall design |
Dermal fibroblasts were isolated from four young (2-months-old) and four old (18-months-old) female C57/Bl6 mice. For sample collection two young and two old mice were sacrificed per day (N=4).From each mouse 5000 fibroblasts were immediately profiled and 5000 fibroblasts were profiled after culturing.
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| Contributor(s) |
Salzer M, Aznar-Benitah S |
| Citation(s) |
30415840 |
| Submission date |
Jul 27, 2018 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
aberenguerl@tauli.cat
|
| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
| Department |
A8G2 Group - Rheumatology Service
|
| Street address |
Parc del Taulí, 1
|
| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (16)
|
| GSM3308381 |
In vivo dermal fibroblasts from young specimen (1214 2018) |
| GSM3308382 |
In vivo dermal fibroblasts from old specimen (1215 2018) |
| GSM3308383 |
In vivo dermal fibroblasts from young specimen (1216 2018) |
| GSM3308384 |
In vivo dermal fibroblasts from old specimen (1217 2018) |
| GSM3308385 |
In vivo dermal fibroblasts from young specimen (1218 2018) |
| GSM3308386 |
In vivo dermal fibroblasts from old specimen (1219 2018) |
| GSM3308387 |
In vivo dermal fibroblasts from young specimen (1220 2018) |
| GSM3308388 |
In vivo dermal fibroblasts from old specimen (1221 2018) |
| GSM3308389 |
Cultured dermal fibroblasts from young specimen (1222 2018) |
| GSM3308390 |
Cultured dermal fibroblasts from old specimen (1223 2018) |
| GSM3308391 |
Cultured dermal fibroblasts from young specimen (1224 2018) |
| GSM3308392 |
Cultured dermal fibroblasts from old specimen (1225 2018) |
| GSM3308393 |
Cultured dermal fibroblasts from old specimen (1226 2018) |
| GSM3308394 |
Cultured dermal fibroblasts from young specimen (1227 2018) |
| GSM3308395 |
Cultured dermal fibroblasts from old specimen (1228 2018) |
| GSM3308396 |
Cultured dermal fibroblasts from young specimen (1229 2018) |
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| This SubSeries is part of SuperSeries: |
| GSE110983 |
The transcriptome of murine dermal fibroblasts |
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| Relations |
| BioProject |
PRJNA483123 |
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