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Series GSE118234 Query DataSets for GSE118234
Status Public on Nov 19, 2018
Title Single cell dissection of Chemical Reprogramming from Fibroblasts into functional Chondrocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used single cell analysis to systematically dissect the sequential changes of cellular phenotypes during fibroblast-chondrocyte direct reprograming.1401 single-cell transcriptomes were measured in total (232 MEFs of day0, 577 chemical-induced intermediate cells (ci-ICs) of day6, 311 ci-chons of day 34, 281 mouse primary chondrocytes (mchons) as positive control). We found that ci-chons and mchons shared similar subpopulation with specifically expression of cartilage-related markers. The findings also indicated that in the early induction, fibroblasts to lose their original features, and transit into a cartilage progenitor intermediate state.
 
Overall design Single cells (MEFs, ci-ICs, ci-chons and primary mouse chondocytes) were captured using FluidigmTM C1 high-throughput IFC. Therefore, the sorted single-cell suspension was loaded on a microfluidic RNA-seq chip. We checked the cells by a microscope for captured cell number confirmation. Then cell lysis, reverse transcription and cDNA pre-amplification were performed on the chip according to Fluidigm’s standard protocol. Illumina libraries were Illumina Nextera XT DNA Sample Preparation kit according to the protocol supplied by Fluidigm. Cell libraries were pooled and sequenced 150 bp paired-end on one lane of Illumina HiSeq xten.
 
Contributor(s) Ouyang H, Chen Y
Citation(s) 32084387, 35477573
Submission date Aug 07, 2018
Last update date May 05, 2022
Contact name Yishan Chen
E-mail(s) YishanChen@intl.zju.edu.cn
Organization name Zhejiang University
Street address #866 Yuhangtang Road
City Hangzhou
ZIP/Postal code 310058
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (1401)
GSM3321821 mchon_1
GSM3321822 mchon_2
GSM3321823 mchon_3
This SubSeries is part of SuperSeries:
GSE118236 High-Resolution Dissection of Chemical Reprogramming from Fibroblasts into Functional Articular Chondrocytes
Relations
BioProject PRJNA485015
SRA SRP156772

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118234_Processed_data_files1.csv.gz 7.6 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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