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Series GSE11851 Query DataSets for GSE11851
Status Public on Sep 01, 2009
Title Gene expression in rat right ventricles during chronic pulmonary embolism
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Pulmonary vascular occlusions due to thromboemboli can result in pulmonary hypertension and right heart damage. Treatments to clear the vascular obstructions such as i.v. heparain or thrombolytics can resolve the hypertension but right ventricular damage often occurs first. Methods of protecting the right ventricle from hypertensive damage during the course of acute treatment to clear the thromboemboli are needed. Monocyte- and neutrophil-mediated inflammation and fibrosis are associated with chronic right ventricular damage but the pathways involved are not understood. A comprehesive survey of gene expression during chronic pulmonary embolism verses control rats has been conducted in this study.
 
Overall design Pulmonary thromoembolism was simulated by injecting rats with 25-micron polystyrene microspheres into the right jugular vein. A dose of 2 million microspheres per 100 grams rat weight was used to generate chronic pulmonary embolism (~350 gram rats; injections ware 0.15 ml/100 gram of a 13 million microsphere per ml suspension in sterile 0.01% Tween-20). Control rats were injected with 0.01 ml/100 grams of Tween-20 "vehicle". After 6 weeks of PE or control, rats were euthanized and hearts surgically excised. Hearts were briefly perfused with buffer to remove blood. Right venticles were removed from whole hearts and separated into "apex" and "outflow-tract" (base) sections. Tisssues were then processed for RNA. More complete descriptions of these methods are available in PMID: 18430806, PMID: 18025228 and PMID: 16814320.
 
Contributor(s) Zagorski J, Obraztsova M, Gellar MA, Kline JA, Watts JA
Citation(s) 19602618, 18808531
Submission date Jun 20, 2008
Last update date Aug 29, 2019
Contact name John Zagorski
E-mail(s) john.zagorski@carolinas.org, j_zagorski@hotmail.com
Phone 704-355-3663
Organization name Carolinas Medical Center
Department Emergency Medicine
Lab Cannon Research Center
Street address 1542 Garden Terrace, 301
City Charlotte
State/province NC
ZIP/Postal code 28203
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (20)
GSM298719 Week 6 vehicle control rat, heart right ventricle apex, biological rep1
GSM298720 Week 6 vehicle control rat, heart right ventricle apex, biological rep2.
GSM298721 Week 6 vehicle control rat, heart right ventricle apex, biological rep3.
Relations
BioProject PRJNA105647

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11851_RAW.tar 52.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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