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| Status |
Public on Dec 31, 2008 |
| Title |
Effect of interferon-gamma on macrophage differentiation and response to Toll-like receptor ligands |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression analysis of freshly isolated CD14+ human monocytes and monocytes cultured in the presence or absence of interferon (IFN) -gamma for 24 h and then stimulated with Pam3Cys, a Toll-like receptor (TLR) 2 ligand, for 6 h. Results provide insight into mechanisms by which IFN-gamma reprograms early macrophage differentiation and subsequent response to TLR ligands.
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| Overall design |
Peripheral blood mononuclear cells were isolated from the blood of two independent healthy human donors by centrifugation on a Ficoll density gradient, and monocytes were positively selected using anti-CD14 magnetic beads. Monocytes were cultured in RPMI 1640 supplemented with 10% fetal bovine serum and 10 ng/mL M-CSF in the presence or absence of 100 U/mL IFN-gamma for 24 h, and then stimulated with 10 ng/mL Pam3Cys for 6 h. Where indicated, RNA was extracted from fresh monocytes immediately upon isolation. Total RNA extraction was performed using TRIzol reagent (Invitrogen) and RNeasy mini kit (Qiagen). Labeled cRNA was prepared with the Affymetrix GeneChip IVT Labeling Kit, and samples were hybridized to Affymetrix HG U133 Plus 2.0 microarrays. Data was processed using Affymetrix protocols and further analyzed using GeneSpring software.
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| Contributor(s) |
Chen J, Ivashkiv LB |
| Citation(s) |
18976936 |
| Submission date |
Jun 23, 2008 |
| Last update date |
Nov 12, 2020 |
| Contact name |
Janice Chen |
| E-mail(s) |
jac2029@med.cornell.edu
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| Phone |
212-774-7188
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| Organization name |
Hospital for Special Surgery
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| Department |
Research
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| Lab |
Lionel Ivashkiv
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| Street address |
535 East 70th St, 4th floor Research
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA105669 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11864_RAW.tar |
217.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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