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| Status |
Public on Sep 30, 2018 |
| Title |
Non-coding RNA, RNY1 is essential for reprogramming process |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
RNY1 is strongly expressed in cytoplasm during early iPS reprogramming phase (d0-3) but, underlying molecular mechanisms of RNY1 remain unknown during iPS reprogramming.
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| Overall design |
TIG-1 fibroblasts was infected using CytoTune-iPS 1.0 Sendai Reprogramming Kit. Cell were transfected siRNA for negative control or RNY1 gene, and harvested at d3 using TRIzol reagent and Directzol kit. We analyzed gene expression changing during early satege of iPS reprogramming.
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| |
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| Contributor(s) |
Kami D, Gojo S |
| Citation(s) |
30273347 |
| Submission date |
Aug 22, 2018 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Satoshi Gojo |
| E-mail(s) |
gojos@koto.kpu-m.ac.jp
|
| Phone |
+81-75-251-5511
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Street address |
465 Kajii-cho, Kamigyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (6)
|
| GSM3349796 |
TIG1 fibroblasts without any treatment_rep1 |
| GSM3349797 |
TIG1 fibroblasts without any treatment_rep2 |
| GSM3349798 |
TIG1 fibroblasts with siNeg & SeV_OSKM_d3_rep1 |
| GSM3349799 |
TIG1 fibroblasts with siNeg & SeV_OSKM_d3_rep2 |
| GSM3349800 |
TIG1 fibroblasts with siRNY1 & SeV_OSKM_d3_rep1 |
| GSM3349801 |
TIG1 fibroblasts with siRNY1 & SeV_OSKM_d3_rep2 |
|
| Relations |
| BioProject |
PRJNA487152 |
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