|
Status |
Public on Dec 26, 2018 |
Title |
STAT2 Signaling Regulates Macrophage Phenotype during Influenza and Bacterial Super-infection |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Influenza is the common respiratory problem that infects between 5-20% of the US population and results in 30,000 deaths annually. A primary cause of the influenza-associated death is due to secondary bacterial pneumonia. In this study, we investigated the role of STAT2 signaling during influenza and influenza-bacterial super-infection in mice. Herein, we demonstrate that STAT2 signaling is required for viral control, regulation of inflammation, and limiting mortality during influenza single infection. Surprisingly, despite this deficiency in anti-viral immunity, we found increased bacterial control and survival in STAT2 deficient mice during influenza-MRSA super-infection compared to controls. This protection in the absence of STAT2 was associated with accumulation of dual phenotype M1/M2 macrophages, which were required for control of bacterial infection. Together, these results suggest that the STAT2 signaling is involved in suppressing macrophage activation and bacterial control during influenza-bacterial super-infection.
|
|
|
Overall design |
We analyzed whole lung RNA from WT and STAT2-/- mice infected with influenza A/PR/8/34 for 6 days followed by MRSA USA300 for one additional day.
|
|
|
Contributor(s) |
Alcorn JF |
Citation(s) |
30337919 |
Submission date |
Aug 24, 2018 |
Last update date |
Mar 25, 2019 |
Contact name |
John F Alcorn |
E-mail(s) |
john.alcorn@chp.edu
|
Organization name |
Children's Hospital of Pittsburgh
|
Department |
Pediatrics
|
Street address |
4401 Penn Ave
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15224 |
Country |
USA |
|
|
Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
Samples (8)
|
|
Relations |
BioProject |
PRJNA487801 |
SRA |
SRP158796 |