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Series GSE119029 Query DataSets for GSE119029
Status Public on Dec 26, 2018
Title STAT2 Signaling Regulates Macrophage Phenotype during Influenza and Bacterial Super-infection
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Influenza is the common respiratory problem that infects between 5-20% of the US population and results in 30,000 deaths annually. A primary cause of the influenza-associated death is due to secondary bacterial pneumonia. In this study, we investigated the role of STAT2 signaling during influenza and influenza-bacterial super-infection in mice. Herein, we demonstrate that STAT2 signaling is required for viral control, regulation of inflammation, and limiting mortality during influenza single infection. Surprisingly, despite this deficiency in anti-viral immunity, we found increased bacterial control and survival in STAT2 deficient mice during influenza-MRSA super-infection compared to controls. This protection in the absence of STAT2 was associated with accumulation of dual phenotype M1/M2 macrophages, which were required for control of bacterial infection. Together, these results suggest that the STAT2 signaling is involved in suppressing macrophage activation and bacterial control during influenza-bacterial super-infection.
 
Overall design We analyzed whole lung RNA from WT and STAT2-/- mice infected with influenza A/PR/8/34 for 6 days followed by MRSA USA300 for one additional day.
 
Contributor(s) Alcorn JF
Citation(s) 30337919
Submission date Aug 24, 2018
Last update date Mar 25, 2019
Contact name John F Alcorn
E-mail(s) john.alcorn@chp.edu
Organization name Children's Hospital of Pittsburgh
Department Pediatrics
Street address 4401 Penn Ave
City Pittsburgh
State/province PA
ZIP/Postal code 15224
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM3356803 WT 1
GSM3356804 WT 2
GSM3356805 WT 3
Relations
BioProject PRJNA487801
SRA SRP158796

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119029_gene_exp.diff.gz 935.2 Kb (ftp)(http) DIFF
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Raw data are available in SRA
Processed data are available on Series record

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