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Series GSE119088 Query DataSets for GSE119088
Status Public on Sep 26, 2018
Title Transcriptomes change differerntly in differernt cancer cells upon EPZ-6438 treatment
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) to find out the difference between EZH2 inhibitor treatment and DMSO group in each cancer cell line, and find the relationship between transcriptomes change and drug sensivitity.
Methods: mRNA profiles of cell lines were generated using Illumina PE150, then GSEA was used for cellular pathways analysis
Results: The result showed 53 common oncogenic signatures were enriched in RNA-seq data. For example, KRAS.300_UP.V1_UP, MEK_UP.V1_UP were statistically enriched in the RNA-seq data. In addition, some oncogenic signatures were only enriched in certain cancer cell lines. For example, 101 and 12 signatures from the RNA-seq were statistically enriched in U2932 and SMMC-7721, respectively.
Conclusions: Our study systematically examined the cellular transcriptomes affected by EPZ-6438, with biologic replicates, generated by RNA-seq technology. EZH2 inhibition results in the transcriptional activation of multiple onco-pathways in a cell-context dependent manner, which may underline the resistance to EZH2 inhibition.
 
Overall design mRNA profiles of 3 cancer cell lines treated with 1 μM EPZ-6348 or DMSO for 6 days, using NEBNext® UltraTM RNA Library Prep it for Illumina® (NEB, USA).
 
Contributor(s) Geng M, Ding J
Citation(s) 30220457
Submission date Aug 27, 2018
Last update date Mar 27, 2019
Contact name Juan Yan
E-mail(s) yanjuan1357@163.com
Phone 15002135233
Organization name Shanghai Institute of Materia Medica
Department Antitumor Pharmacology
Lab Meiyu Geng
Street address 555 Zuchongzhi Road
City Shanghai
ZIP/Postal code 201203
Country China
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (18)
GSM3357696 pfeif_c1
GSM3357697 pfeif_c2
GSM3357698 pfeif_c3
Relations
BioProject PRJNA488071
SRA SRP158917

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Supplementary file Size Download File type/resource
GSE119088_S7721_EvsS7721_c_DEG.xls.gz 2.7 Mb (ftp)(http) XLS
GSE119088_U2932_EvsU2932_c_DEG.xls.gz 2.7 Mb (ftp)(http) XLS
GSE119088_pfeif_Evspfeif_c_DEG.xls.gz 2.7 Mb (ftp)(http) XLS
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