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| Status |
Public on Sep 04, 2018 |
| Title |
RNA-Seq Analysis of Wild Type and LGMN-/- Peritoneal Macrophage Transcriptomes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Research on the interaction between tumour-associated macrophages (TAMs) and tumour cells has generated great interest in the potential use of macrophages as a therapeutic strategy against tumour cells. Here, we show that deletion of legumain, a conversed proteolytic enzyme in macrophages, promotes senescence of macrophages and tumour cells. Peritoneal macrophages isolated from wide type and lgmn-/- mice were co-cultured with EO771 breast cancer cells for 4 days. Total RNA was extracted for RNA-Seq analysis, cDNA library construction and sequencing were performed using BGISEQ-500 platform.
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| Overall design |
Examination of differential expressive genes in peritoneal macrophages extracted from wide type and lgmn-/- mice after co-cultured with EO771 cells for 4 days.
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| Contributor(s) |
Shen L, Tan X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 03, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Long Shen |
| E-mail(s) |
shenlong@tmu.edu.cn
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| Phone |
15202209080
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| Organization name |
School of Basic Medical Sciences, Tianjin Medical University
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| Street address |
22 Qixiangtai Road
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| City |
Tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA489095 |
| SRA |
SRP159470 |
