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Status |
Public on Jun 21, 2019 |
Title |
The role of TERF2IP (Telomeric repeat binding factor 2-interacting protein) in regulating disturbed flow-induced changes of gene expression |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To understand the function of TERF2IP in ECs, we isolated total RNAs from human aortic endothelial cells (HAECs) transfected with control or TERF2Ip siRNA and transcriptionally profiled them.
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Overall design |
HAECs were transfected by control siRNA or TERF2IP siRNA, and after 48 hrs of transfection HUVECs were exposed to disturbed flow (d-flow). After 24 hrs of disturbed flow RNA was extracted for futher microarray processing. We have the following four groups (1. Control siRNA under static condition (siCont), 2. Control siRNA under d-flow (siCont + DF), 3. TERF2IP siRNA under d-flow (siTERF2IP), and 4. TERF2IP siRNA under d-flow (siTERF2IP + DF), and each group have three samples and total twelve samples were analyzed.
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Contributor(s) |
Abe J, Le N, Lusis AJ |
Citation(s) |
31045573 |
Submission date |
Sep 04, 2018 |
Last update date |
Jun 21, 2019 |
Contact name |
Jun-ichi Abe |
E-mail(s) |
jabe@mdanderson.org
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Phone |
5854745456
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Organization name |
University of Texas MD Anderson Cacer Center
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Street address |
2121 W Holcombe Blvd. IBT8 803e
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City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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Samples (12)
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Relations |
BioProject |
PRJNA489190 |