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| Status |
Public on Sep 01, 2019 |
| Title |
Transcriptome analysis of Y79 retinoblastoma cells upon Lysyl oxidase propeptide (LOX-PP) overexpression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Lysyl oxidase (LOX) is an copper dependent amine oxidase enzyme involved in cross linking of collagens and elastins. Lysyl oxidase propeptide (LOX-PP) is 18 kDa region cleaved during maturation of LOX and its anti-tumorigenic role were studied in various cancers. The effect of LOX-PP overexpression in retinoblastoma cancer cells (Y79) using transient transfection of LOX-PP gene accessed for global gene deregulations. The analysis resulted in RB cancer cell death through deregulation of retinoblastoma in cancer, cell cycle, apoptosis, focal adhesion-PI3K-AKT signaling and DNA repair mechanism pathway. Further validations were done using RT-PCR and western blot analysis. Our results provide evidence that inducing apoptosis through AKT-NFκB signaling.
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| Overall design |
We analyzed Y79 cell from 2 Empty vector and 2 LOX-PP overexpressed cells using the Affymetrix Human Transcriptome array 2.0 platform. Array data was processed by Affymetrix Transcriptome Analysis Console (TAC) 4.0 - Exon level Array Computational Tool. No techinical replicates were performed.
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| Contributor(s) |
Ragavachetty Nagaraj N, Konerirajapuram Natarajan S, Karunakaran C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 04, 2018 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Karunakaran Coral |
| Organization name |
Vision Research Foundation, Chennai
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| Department |
R S Mehta Jain Department of Biochemistry and Cell Biology,
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| Street address |
41, College road
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| City |
Chennai |
| State/province |
Tamilnadu |
| ZIP/Postal code |
600006 |
| Country |
India |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA489199 |
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