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Series GSE119441 Query DataSets for GSE119441
Status Public on Sep 02, 2020
Title The effects of perfluorooctanoate on high fat diet induced non-alcoholic fatty liver disease in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We reported the hepatic gene expression profiling in mice treated by perfluorooctanoate (PFOA) and high fat diet (HFD). Chronic HFD treatment was associated with gene expression changes in cholesterol biosynthetic process, lipid metabolic process, extracellular matrix, and inflammatory response pathways. Many chemokine related genes including Ccl2, Ccr2, Ccl3l3, Cx3cl1, Cx3cr1, Cxcl14, and toll-like receptor (TLR) related genes including Tlr2, Tlr7, Tlr8, Tlr13 were all significant upregulated comparing vehicle-treated HFD-fed mice to control diet (CD)-fed mice, suggesting their roles in the development of steatohepatitis. PFOA induced gene expression changes in PPAR signaling, fatty acid degradation, biosynthesis of unsaturated fatty acids, and chemical carcinogenesis pathways regardless of diet. However, we showed preexisting fatty liver enhanced the lipid clearance effect of PFOA compared to that in a normal liver. At last, chronic exposure to HFD and PFOA was found to interact on genes related to PPAR signaling, chemical carcinogenesis, and ABC transporter pathways.
 
Overall design Two groups of mice were fed with either a control or high-fat diet for 16 weeks to induce normal or steatotic livers, respectively. Subsequently, mice from each diet group were treated with PFOA or vehicle for another 8 weeks. Each mouse was considered a biological replicate. A total of 4 groups with 4 mice in each group were used.
 
Contributor(s) Li X, Wang Z, Podicheti R, Klaunig JE
Citation(s) 30711707
Submission date Sep 04, 2018
Last update date Dec 02, 2020
Contact name Xilin Li
E-mail(s) lixilin0826@gmail.com
Phone 8624858599
Organization name Indiana University
Street address 2719 E 10th street Rm230
City Bloomington
State/province Indiana
ZIP/Postal code 47408
Country USA
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (16)
GSM3374696 JEK16218_CD + Vehicle
GSM3374697 JEK16219_CD + Vehicle
GSM3374698 JEK16220_CD + Vehicle
Relations
BioProject PRJNA489258
SRA SRP159531

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Supplementary file Size Download File type/resource
GSE119441_genecounts.txt.gz 1.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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