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| Status |
Public on Apr 16, 2019 |
| Title |
Single-Cell Transcriptomics Identifies TOX as a Key Transcriptional Regulator of Progenitor-Like CD8 T Cells in Chronic Infection [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stem-like CD8 T cells maintain long-term antiviral CD8 immunity during chronic infection, and share regulatory pathways with memory precursor effector cells generated after acute infection. However, it is unclear whether stem-like CD8 T cells require distinct transcriptional and epigenetic regulation for their longevity and adaptation to the immunosuppressive environment in chronic infection. Here, our comparison of single-cell transcriptomes and epigenetic profiles of CD8 T cells responding to acute and chronic viral infections revealed that stem-like CD8 T cells became distinct from memory precursors before clonal expansion ended. We found that a coexpression gene module containing Tox exhibited higher transcriptional activities and active histone marks in stem-like T cells than memory precursors. Moreover, TOX promoted persistence of antiviral CD8 T cells, and was required for stem-like CD8 differentiation. Our results indicate that stem-like CD8 T cells require unique transcriptional and epigenetic programs for their differentiation and persistence during chronic viral infection.
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| Overall design |
Single-cell tcranscriptomic and epigentic analysis of antigen specific CD8 T cells after acute and chronic viral infection. Transcriptome analysis of TOX over expression in antigen specific CD8 T cells after chronic viral infection.
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| Contributor(s) |
Yao C, Sun HW, O'Shea JJ, Schwartzberg PL, Wu TQ |
| Citation(s) |
31209400 |
| Submission date |
Sep 13, 2018 |
| Last update date |
Jul 03, 2019 |
| Contact name |
Tuoqi Wu |
| E-mail(s) |
tuoqiwu@gmail.com
|
| Organization name |
National Institute of Allergy and Infectious Diseases
|
| Street address |
49 Convent Dr.
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE119943 |
Single-Cell Transcriptomics Identifies TOX as a Key Transcriptional Regulator of Progenitor-Like CD8 T Cells in Chronic Infection |
|
| Relations |
| BioProject |
PRJNA490747 |
| SRA |
SRP161707 |
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