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| Status |
Public on Sep 18, 2018 |
| Title |
Stochastic profiling of matrix-attached MCF10A-5E cells in 3D culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The objective of this study was to profile the gene-regulatory heterogeneities of breast epithelial cells grown in an ECM-rich environment. The approach was to use stochastic profiling (Janes et al., Nat Methods 7(4):311-7 [2010]) to collect 10-cell pools of ECM-attached cells obtained by laser-capture microdissection and measure fluctuations in the 10-cell-averaged measurements to infer heterogeneous regulatory states.
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| Overall design |
16 random 10-cell samples of ECM-attached MCF10A-5E cells were obtained by laser-capture microdissection and their expression fluctuations compared to 16 pool-and-split controls comprised of larger microdissected cell populations extracted and split into 10-cell equivalents before poly(A) PCR preamplifcation.
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| Contributor(s) |
Janes KA, Brugge JS |
| Citation(s) |
20228812 |
| Submission date |
Sep 17, 2018 |
| Last update date |
Sep 18, 2018 |
| Contact name |
Kevin A. Janes |
| E-mail(s) |
kjanes@virginia.edu
|
| Organization name |
University of Virginia
|
| Department |
Department of Biomedical Engineering
|
| Street address |
Box 800759 Health System
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
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| Platforms (1) |
| GPL6255 |
Illumina humanRef-8 v2.0 expression beadchip |
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| Samples (32)
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| GSM3391861 |
10-cell pool-and-split control replicate 1 |
| GSM3391862 |
10-cell pool-and-split control replicate 2 |
| GSM3391863 |
10-cell pool-and-split control replicate 3 |
|
| Relations |
| BioProject |
PRJNA491420 |
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