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| Status |
Public on Jul 10, 2008 |
| Title |
Modulation of colon inflammation in interleukin-10 gene-deficient mice via dietary polyunsaturated fatty acid |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Dietary n-3 polyunsaturated fatty acids can reduce inflammation via a range of mechanisms. This study tested the effect of dietary eicosapentaenoic acid (EPA) on intestinal inflammation using interleukin-10 gene-deficient (Il10-/-) mice. Methods: At 35 days of age, 12 weaned Il10-/- and 12 C57 mice were randomly assigned to one of two modified AIN-76A diets, supplemented with 3.7% purified ethyl esters of either EPA (n-3) or oleic acid (OA, control). To identify genes relevant to colon inflammation, transcription profiling (microarrays and qRT-PCR) and bioinformatic analyses were used. Results: In this study, dietary EPA reversed the decrease in colon fatty acid β-oxidation gene expression observed in OA-fed Il10-/- compared to C57 mice. Il10-/- mice fed the OA diet showed decreased expression of antioxidant enzyme genes, as well as those involved in detoxification of xenobiotics, compared to C57 mice on the same diet. In contrast, dietary EPA increased the expression of these genes in Il10-/- mice. Conclusions: These data indicate that dietary EPA induced endogenous lipid oxidation which might have a potential anti-inflammatory effect on colon tissue. This is supported by the activation of the Ppara gene that regulates the expression of pro-inflammatory and immunomodulatory genes and proteins.
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| Overall design |
The diet abbreviations EPA, OA, AA and CO used in the sample records
refer to the following :
CO : AIN-76A (control)
OA : AIN-76A (fat-free) + 1% corn oil + 3.7% oleic acid
EPA : AIN-76A (fat-free) + 1% corn oil + 3.7% eicosapentaenoic acid
AA : AIN-76A (fat-free) + 1% corn oil + 3.7% arachidonic acid
Corn oil was supplemented with purified linoleic and alpha-linolenic acid to meet the nutritional requirements of mice for these essential fatty acids. Diets fed for 6 weeks.
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| Contributor(s) |
Knoch B |
| Citation(s) |
20440721 |
| Submission date |
Jul 07, 2008 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Alan Francis McCulloch |
| E-mail(s) |
alan.mcculloch@agresearch.co.nz
|
| Phone |
64 3 4899080
|
| Organization name |
AgResearch NZ
|
| Street address |
Invermay
|
| City |
Puddle Alley |
| State/province |
Mosgiel |
| ZIP/Postal code |
n/a |
| Country |
New Zealand |
| |
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| Platforms (1) |
| GPL2872 |
Agilent-012694 Whole Mouse Genome G4122A (Feature Number version) |
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| Samples (16)
|
| GSM303588 |
C57 mice AA diet colon tissue (Pool 1) |
| GSM304030 |
C57 mice AA diet colon tissue (Pool 2) |
| GSM304042 |
IL10-/- mice AA diet colon tissue (Pool 1) |
| GSM304043 |
IL10-/- mice AA diet colon tissue (Pool 2) |
| GSM304044 |
C57 mice control diet colon tissue (Pool 1) |
| GSM304045 |
C57 mice control diet colon tissue (Pool 2) |
| GSM304046 |
IL10-/- mice control diet colon tissue (Pool 1) |
| GSM304047 |
IL10-/- mice control diet colon tissue (Pool 2) |
| GSM304048 |
C57 mice EPA diet colon tissue (Pool 1) |
| GSM304049 |
C57 mice EPA diet colon tissue (Pool 2) |
| GSM304050 |
IL10-/- mice EPA diet colon tissue (Pool 1) |
| GSM304051 |
IL10-/- mice EPA diet colon tissue (Pool 2) |
| GSM304052 |
C57 mice OA diet colon tissue (Pool 1) |
| GSM304053 |
C57 mice OA diet colon tissue (Pool 2) |
| GSM304054 |
IL10-/- mice OA diet colon tissue (Pool 1) |
| GSM304055 |
IL10-/- mice OA diet colon tissue (Pool 2) |
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| Relations |
| BioProject |
PRJNA113305 |
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