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| Status |
Public on Nov 15, 2018 |
| Title |
TNF response in promyelocytic and granulocytic forms of HL60/S4 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The human promyelocytic cell line HL60/S4 can be differentiated into a granulocytic form with the addition of all-trans retinoic acid for 4 days. We investigated the conserved and differentiated responses to TNF in the granulocytic and promyelocytic forms of HL60/S4 cells.
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| Overall design |
Promyeloytic and granulocytic HL60/S4 cells were treated with 16ng/ml TNF or equivalent volume of vehicle (water) in calcium supplemented growth media for two hours, then RNA was extracted with Trizol LS and the Qiagen Rneasy mini kit.
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| Contributor(s) |
Jacobson E, O'Sullivan J |
| Citation(s) |
31263060 |
| Submission date |
Sep 27, 2018 |
| Last update date |
Aug 21, 2019 |
| Contact name |
Justin M. O'Sullivan |
| E-mail(s) |
justin.osullivan@auckland.ac.nz
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| Organization name |
University of Auckland
|
| Department |
Liggins Institute
|
| Street address |
85 Park Road, Grafton
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| City |
Auckland |
| ZIP/Postal code |
1023 |
| Country |
New Zealand |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA493630 |
| SRA |
SRP162760 |
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