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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 03, 2018 |
| Title |
Screening for potential target genes of microRNA-548ac using HTA 2.0 microarrays |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We screened for target genes of hsa-miR-548ac by transcriptome profiling. The miRNA was overexpressed in HeLa cells by transient transfection of a precursor miRNA expression plasmid. High-density Affymetrix Human Transcriptome Arrays (HTA) 2.0 were used for the gene expression analysis. This GEO entry provides the microarray data preprocessed according to the gene level workflow.
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| Overall design |
HeLa cells were transiently transfected with a non-viral precursor miRNA expression plasmid (HmiR1085-MR04, GeneCopoeia). As negative control, a plasmid with scrambled sequence (CmiR0001-MR04, GeneCopoeia) was used. RNA samples were obtained 24 h and 48 h after transfection of the mir-548ac plasmid (n=3 replicates) or the control plasmid (n=2 replicates). For all 10 RNA samples, transcript levels were measured with Affymetrix HTA 2.0 microarrays.
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| Contributor(s) |
Hecker M, Koczan D, Boxberger N, Zettl UK |
| Citation(s) |
30730892 |
| Submission date |
Oct 02, 2018 |
| Last update date |
Feb 12, 2019 |
| Contact name |
Michael Hecker |
| E-mail(s) |
michael.hecker@rocketmail.com
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| Organization name |
University of Rostock
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| Department |
Department of Neurology
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| Lab |
Division of Neuroimmunology
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| Street address |
Gehlsheimer Str. 20
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| City |
Rostock |
| ZIP/Postal code |
18147 |
| Country |
Germany |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (10)
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| GSM3414971 |
negative control, 24 h post-transfection, replicate 1 |
| GSM3414972 |
negative control, 24 h post-transfection, replicate 2 |
| GSM3414973 |
negative control, 48 h post-transfection, replicate 1 |
| GSM3414974 |
negative control, 48 h post-transfection, replicate 2 |
| GSM3414975 |
miR-548ac overexpression, 24 h post-transfection, replicate 1 |
| GSM3414976 |
miR-548ac overexpression, 24 h post-transfection, replicate 2 |
| GSM3414977 |
miR-548ac overexpression, 24 h post-transfection, replicate 3 |
| GSM3414978 |
miR-548ac overexpression, 48 h post-transfection, replicate 1 |
| GSM3414979 |
miR-548ac overexpression, 48 h post-transfection, replicate 2 |
| GSM3414980 |
miR-548ac overexpression, 48 h post-transfection, replicate 3 |
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| Relations |
| BioProject |
PRJNA494420 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE120769_RAW.tar |
223.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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