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| Status |
Public on Oct 04, 2018 |
| Title |
Single cell RNA-sequencing of innate lymphoid cells isolated from mouse skin layers |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Determine the layer-specific indentities and heterogeneity of innate lymphoid cells in the skin by single cell transcriptomic analysis
Methods: Innate lymphoid cells were sorted from epidermis, dermis and subcutis of adult B6 mice and subjected to droplet based single-cell captureing and RNA isolation and library preparation.
Results: 3,431, 3,356 and 1,061 innate lymphoid cells from epidermis, dermis, and subcutis were analyzed. Unsupervised clustering of the combined data set performed in Seurat identified 6 distinct ILC clusters.
Conclusions: This study reveals skin layer-specific transcriptomic signature of innate lymphoid cells
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| Overall design |
Transcriptome profiles of skin innate lymphoid cells were generated by droplet-based single cell RNA-seq
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| Contributor(s) |
Kobayashi T, Voisin B, Nagao K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 03, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Tetsuro Kobayashi |
| Organization name |
NIAMS/NIH
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm12N256
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA494536 |
| SRA |
SRP163240 |
