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| Status |
Public on Apr 05, 2019 |
| Title |
Histological Chorioamnionitis Induces Differential Gene Expression in Human Cord Blood Mononuclear Leukocytes from Term Neonates |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human transcriptome array analysis of human cord blood mononuclear leokocytes from neonates exposed to histological chorioamnionitis and compared with healthy neonates
Histological chorioamnionitis (HCA) is an infection of fetal membranes and complicates 5.2% to 28.5% of all live births. Exposure to HCA may have long-term consequences including an increased risk for allergic disorders and asthma later in childhood, the mechanism(s) of which are still not yet well understood. Our objective was to determine the mRNA transcriptome of cord blood mononuclear leukocytes from term neonates and identify key genes and pathways involved in HCA. We found several key genes differentially expressed with exposure to HCA. These transcriptomes included novel genes and pathways associated with exposure to HCA. The differential gene expression included the key genes regulating inflammatory, immune, respiratory and neurological pathways, which may contribute to disorders in those pathways in neonates exposed to HCA.
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| Overall design |
We analyzed gene expression from cord blood mononuclear leukocytes (5 histological chorioamnionitis exposed neonates and 5 healthy control neonates) using the Affymetrix Human transcriptome array 2.0. Array data was processed by Affymetrix Array Computational Tool.
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| Contributor(s) |
Gayen nee’ Betal S, Murthy S, Favara M, Fong G, Chan JS, Addya S, Shaffer TH, Greenspan J, Bhandari V, Rahman I, Aghai ZH |
| Citation(s) |
30971730 |
| Submission date |
Oct 04, 2018 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Zubair H Aghai |
| E-mail(s) |
Zubair.Aghai@nemours.org
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| Phone |
2159556523
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| Organization name |
Thomas Jefferson University/ Nemours
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| Department |
Pediatrics/ Neonatology
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| Lab |
Neonatology Research Lab
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| Street address |
833 Chestnut Street, Suite 1201
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA494716 |
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